MEFs (Oct4-GFP)

MEFs (Oct4-GFP)
Model: PCEMM03
Species: Mus Musculus
Catalog Number: PCEMM03
Availability: In Stock
Price: $549.00
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Species: Mus Musculus (129/B6 Mix) Catalog Number: PCEMM03
Cell Number: ~1x106 Storage/Shipping: Dry Ice

Introduction:

Primary mouse embryonic fibroblasts (MEFs) are typically derived from E13.5 mouse embryos. They are relatively easy to culture and maintain and have robust proliferation rate. MEFs can be powerful tools to study gene functions especially when comparison experiments between wild-type and knockout cells from the same liter are required. Meanwhile, MEFs have also been used as feeder cells to support growth of embryonic stem cells from a variety of species1. More recently, MEFs from reporter transgenic mice were used for assessing gene function during reprogramming of fibroblasts to induced pluripotent stem cells (iPSCs) 2.

PrimCells is proud to isolate and provide the primary cultures of embryonic fibroblasts from transgenic mice carrying IRES-GFP gene in their endogenous Pou5f1 (Oct4) locus. When these cells are fully reprogrammed, iPSCs will become GFP+, which could be a powerful tool for chemical screening or gene functional studies in order to develop more efficient reprogramming technology.

Figure 1 | Successfully reprogrammed iPSCs become GFP positive

 

Click here to download PDF datasheet

 

For additional information regarding the product and technical questions, please contact Supports@PrimCells.com.  You are guaranteed to receive a response within 24hrs from one of our scientists.

1. Thomson, J.A. et al. Embryonic stem cell lines derived from human blastocysts. Science 282, 1145-1147 (1998).

2. Mikkelsen, T.S. et al. Dissecting direct reprogramming through integrative genomic analysis. Nature 454, 49-55 (2008).

This product is intended for laboratory research purposes only. It is not intended for use in humans. While PrimCells uses reasonable efforts to include accurate and up-to-date information on this product sheet, PrimCells makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. PrimCells does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. PrimCells is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, PrimCells is not liable for damages arising from the misidentification or misrepresentation of cultures.
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