Mouse Oct4-GFP ES Cells

Mouse Oct4-GFP ES Cells
Model: PCEMM08
Species: Mouse
Catalog Number: PCEMM08
Availability: In Stock
Price: $899.00
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Oct4 is a transcriptional factor from POU protein family. It is one of the most important markers for undifferentiated embryonic stem cells and is required to maintain the self-renwal of mESCs1. Oct4 is also associated with human germ cell tumors2.

PrimCells is proud to isolate and provide to the research community the culture of Oct4-EGFP ESCs from transgenic knock-in mice. These cells were cultured in standard ESCs medium on gelatin coated plates and be used as a powerful tool to examine the mechanism of self-renwal of mouse embryonic stem cells. Oct4-GFP mESCs should be maintained in the stem cell culture medium with LIF to maintain the pluripotent state (Figure 1).

Note: This cell line is puromycin-resistent. 

Oct4-GFP mESC

Figure 1. Oct4-GFP mESCs show GFP signals when maintained in ESC medium (Left). The differentiated cells lose the GFP signals in the absence of LIF (Right).

 

References

1.   Loh, Y.H. et al. The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. Nat Genet 38, 431-440 (2006).

2.   Looijenga, L.H. et al. POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors. Cancer Res 63, 2244-2250 (2003).

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For additional information regarding the product and technical questions, please contact Supports@PrimCells.com.  You are guaranteed to receive a response within 24hrs from one of our scientists.Supports@PrimCells.com

1. Loh, Y.H. et al. The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. Nat Genet 38, 431-440 (2006).
2. Looijenga, L.H. et al. POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors. Cancer Res 63, 2244-2250 (2003).
 

This product is intended for laboratory research purposes only. It is not intended for use in humans. While PrimCells uses reasonable efforts to include accurate and up-to-date information on this product sheet, PrimCells makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. PrimCells does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. PrimCells is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, PrimCells is not liable for damages arising from the misidentification or misrepresentation of cultures.

© PrimCells 2013. All rights reserved. 

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