Mouse Sox1-GFP ES cells

Mouse Sox1-GFP ES cells
Model: PCEMM01
Species: Mus musculus
Catalog Number: PCEMM01
Availability: In Stock
Price: $899.00
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Species: Mus Musculus Catalog Number: PCEMM01
Cell Number: ~2x106 Storage/Shipping: Dry Ice

 

Introduction:
 
Sox1 is a transcriptional factor from SOX protein family. It is one of the earliest and most specific markers for neural progenitors and expressed throughout the central neural system during early embryo development1, 2. PrimCells is proud to isolate and provide to the research community the culture of Sox1-EGFP ESCs from transgenic knock-in mice. These cells were cultured in standard ESCs medium on gelatin coated plates and be used as a powerful tool to examine the process by which mESCs acquire neural identity.
 

ES cell culture and monolayer neural differentiation procudures

Sox1-GFP mESCs should be maintained in the stem cell culture medium to keep the pluripotent state (Figure 1).

For monoculture neural differentiation, dissociate and plate the undifferentiated Sox1-GFP mESCs  on to 0.1% gelatin-coated tissue culture plastic at a density of 1-3x104/cm2 in serum-free N2B27 medium. Renew N2B27 medium every 2 days. GFP positive cells can be observed on Day 5 (Figure 2).

 
Figure 1 | Sox1-GFP mESCs express typical ES cell markers

 

Figure 2 |  Monoculture neuronal differentiation of Sox1-GFP mESCs on Day 5

Growth Medium

Embryonic stem cell medium

DMEM:   415ml

FBS: 75ml (HYCLONE LABORATORIES INC, Cat# SH30070.03EH)

L-Glutamine: 5ml

MEM NEAA: 5ml

1-Thioglycerol: 4.3ul

LIF: 50ul LIF: 50ul (10ng/ml final concentration)  (PrimCells, Cat# PFHSA01)

Total Volume: 500ml

N2B27 medium for neural differentiation

N2B27 is a 1:1 mixture of DMEM/F12 (Gibco, Paisley, UK) supplemented with modified N2 (25 µg/ml insulin, 100 µg/ml apo-transferrin, 6 ng/ml progesterone, 16 µg/ml putrescine, 30 nM sodium selenite and 50 µg/ml bovine serum albumin fraction V (Gibco)) and Neurobasal medium supplemented with B27 (both from Gibco).

Alternatively, Ndiff227 (StemCells INC, Cat# SCS-SF-NB-02) is ready-to-use medium for the neural differentiation of mESCs  in adherent monoculture conditions as described in Ying QL et al (2003)2.

References

1.   Aubert, J. et al. Screening for mammalian neural genes via fluorescence-activated cell sorter purification of neural precursors from Sox1-gfp knock-in mice. Proc Natl Acad Sci U S A 100 Suppl 1, 11836-11841 (2003).

2.   Ying, Q.L., Stavridis, M., Griffiths, D., Li, M. & Smith, A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat Biotechnol 21, 183-186 (2003).

 

Click here to download PDF datasheet

 
For additional information regarding the product and technical questions, please contact Supports@PrimCells.com.  You are guaranteed to receive a response within 24hrs from one of our scientists.

1. Aubert, J. et al. Screening for mammalian neural genes via fluorescence-activated cell sorter purification of neural precursors from Sox1-gfp knock-in mice. Proc Natl Acad Sci U S A 100 Suppl 1, 11836-11841 (2003).
2. Ying, Q.L., Stavridis, M., Griffiths, D., Li, M. & Smith, A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat Biotechnol 21, 183-186 (2003).

This product is intended for laboratory research purposes only. It is not intended for use in humans. While PrimCells uses reasonable efforts to include accurate and up-to-date information on this product sheet, PrimCells makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. PrimCells does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. PrimCells is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, PrimCells is not liable for damages arising from the misidentification or misrepresentation of cultures.
© PrimCells 2013. All rights reserved.

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